Isolation of Casein

Experiment you: Isolation of Casein from Milk

5. pH of milk – 6. 6

* milk = 87. 1% drinking water, 4. being unfaithful % CHO 3. being unfaithful %, excess fat, 0. seven percent minerals Test 2: Proteins Hydrolysis and Characterization

| Reagents| Principle| Check for| Confident Result| Bad Result| Biuret | CuSO4, NaOH| Complexation of Cu+2 with amide N atoms| Polypeptide bonds| Violet/purple solution| Blue color solution| Sakaguchi| 10% NaOH, 0. 02% α-naphtol answer, 2% NaOBr| arginine condenses with α-naphtol and NaOH| Guanido group (arginine)| orange colored solution| mild yellow solution| Ninhydrin| zero. 1% ninhydrin solution (1, 2, 3-indanetrione monohydrate as well as triketohydrindene hydrate)| Oxidative deamination and decarboxylation; reduction of ninhydrin| free of charge α-amino and carboxyl groups| purple-blue color solution| Yellow solution| Xanthoproteic| Conc. HNO3, 50% NaOH| Nitration alternative in Benzene ring| aromatic amino acids| yellow medications then turns orange once neutralized with NaOH| obvious solution, zero change in color| Hopkins-Cole| Hopkins-Cole reagent| Condensation of natura group with glyoxylic acidity and H2SO4| tryptophan| Purple ring with the junction where two levels meet| Lack of purple ring| * Chemical p hydrolysis is known as a better process than Alkaline hydrolysis because of the less destruction of amino acids Experiment several: Protein Assay by Liverpool Method

5. Coomassie Brilliant Blue G-250

* Bradford Reagent

5. Bovine Serum Albumin sama dengan BSA

* Reagent write off = test tube #1 = 0mL BSA inventory solution, 1mL distilled INGESTING WATER * 595nm

* Story A595 vs concentration of BSA

Test 4: Enzymatic Activity of Salivary Amylase

* Iodine test out – presence of starch

* 1/t vs temperature/pH

Experiment 5: General and Specific Checks for Carbohydrates

| Reagents| Principle| Test for| Positive Result| Negative Result| Molisch| Molisch Reagent, Conc. H2SO4| hydrolysis, dehydration building either a furfural or a 5-hydroxufurfural; condensation...



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